ASTM D5712 PDF

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ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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Latex processed without silica, does have protein residues, see Table II. Please login to your authorized staff account to use this feature.

One is to protect the cured latex from sticking to itself during manufacturing process, and the other is to provide internal lubricity while donning the gloves.

At least two specific tests were developed to further evaluate allergenic protein levels. FORMAT Single User Subscription Site License Subscription BOOKS All Books PACKAGES All Packages EDITION to PUBLISHER. Washing is minimal and since the latex is clean, there is no need for costly off line post treatments. R5712 III shows results with silica at a lower level ast 0. A non-silica rubber blank has micrograms of proteins per gram of rubber, see Table I.

November 20, Content source: D Specification for Rubber Surgical Asstm. If you like to setup a quick demo, let us know at support madcad. Total and specific analysis of proteins in latex goods The total quantity of d57122 proteins in gloves is evaluated by extracting the rubber with an aqueous solution and measuring according to ASTM D No items in cart.

It then could be concluded that the latex has been cleaned. This problem has become more apparent in the last several years due to the increased use of rubber gloves for infectious diseases protection. Print 1 2 3 4 5 page sstarting from page current page.

Introduction The problem of proteins in natural rubber Type I latex allergies can be caused by proteins in the natural latex used in gloves, condoms, balloons and other articles.

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Tables I and II report results with silica at a level of 1 to 5 weight percentage. Register for a aetm account. Astmm previous paper on cleaning latex films with fumed silica5 presented protein results derived by ASTM D Analytical information demonstrated that powdered latex gloves were found to contain high levels of protein between 1 and micrograms of protein per gram of rubber.

Here again, the presence of silica greatly diminished the level of latex allergenic proteins measured by the LEAP method.

ASTM-D, –

It was found that the addition of fumed silica to latex compounds helps in manufacturing of cleaner latex goods with lower protein extractables. Thompson et al9 that there is a relationship between hydration and compromised barriers in some surgical gloves. Link to Active This link will always route to the current Active version of the standard. The proteins probably would stay adsorbed on the latex particles throughout the compounding process.

According to the literature,8 when the latex is in the tree, the particles are presumed to possess an adsorbed layer of proteins—lipid complex. This problem has been publicized in the media and described in the literature.

Latex protein analysis ASTM D, might not represent a true picture of the latex irritation problem. Experimental results and discussion Low-protein allergen analysis in latex compounded with fumed silica Compounding of fumed silica dispersion for dipped natural latex application resulted in tensile and tear enhancement as described before.

Contrary to conventional thickening agents, silica enhances the tensile. Two different sources of blood serum were used. While donning and removing powdered gloves, the protein-coated particles become air borne dust and can contaminate large volumes.

An additional advantage is enhancing latex viscosity resulted only with fumed silica B.

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The proteins then easily are removed. Other methods astk protein removal treatment include passing the latex through special ion exchange resins6 or altering the protein structure with protease enzymes. It is suggested by R. Allergen units can range from 10, units per ml for an d571 from raw latex film to 1 unit per ml extracted from vinyl glove film negative control or latex with undetectable level of allergenic proteins.

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Today, some 12, ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. This is in contradiction to what was proposed before where fumed silica attaches itself to the proteins on the surface of the latex particle, binding them there. Allergy and Clinical Immunology, 58, It is reported that surgical gloves, long assumed effective barriers against blood-born pathogens, may not be effective as originally thought and, further, improperly manufactured gloves may cause a serious threat to healthcare workers and patients.

Potential hazards include skin absorption of harmful substances, chemical or thermal burns, electrical dangers, bruises, abrasions, cuts, punctures, fractures and amputations. November 20, Page last updated: Asrm extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

Are you sure you want to empty the cart? The silica also may serve as a thickening agent. Historical Version s – view previous versions of standard. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination.

The following editions for this book are also available Adkinson, Journal of Allergy and Clinical Immunology, 98, Examples of test methods include, but are not limited to: Some laboratories claim that they can measure lower levels of proteins.

Hamilton, Journal of Allergy and Clinical Immunology, 96, ,